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Article summary:
1. The Rnf complex of Clostridium ljungdahlii is a proton-translocating ferredoxin:NAD+ oxidoreductase essential for autotrophic growth.
2. Disruption of the C. ljungdahlii rnf operon inhibited autotrophic growth, resulting in a collapse of ATP synthesis, proton gradient, membrane potential, and proton motive force.
3. The Rnf complex is also implicated in nitrogen fixation and is responsible for translocating protons across the membrane to elicit energy conservation during acetogenesis.
Article analysis:
The article provides evidence that the Rnf complex of Clostridium ljungdahlii is a proton-translocating ferredoxin:NAD+ oxidoreductase essential for autotrophic growth by demonstrating that disruption of the C. ljungdahlii rnf operon inhibited autotrophic growth, resulting in a collapse of ATP synthesis, proton gradient, membrane potential, and proton motive force. The article also suggests that the Rnf complex is implicated in nitrogen fixation and is responsible for translocating protons across the membrane to elicit energy conservation during acetogenesis.
The article appears to be reliable as it provides evidence to support its claims and does not appear to be biased or one-sided in its reporting. It does not make unsupported claims or omit any points of consideration or evidence for its claims made. It does not appear to contain any promotional content or partiality towards any particular point of view or opinion. Possible risks are noted throughout the article and both sides are presented equally with no unexplored counterarguments present.