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1. RNA aptamers: small-molecule fluorophores (SFs) systems have emerged as novel single mRNA imaging probes since 2019, providing advantages such as fluorogenic ability and minimal perturbation.

2. This review summarizes the five reported RNA aptamers: SFs systems for single mRNA imaging in living cells so far, highlighting their potential for studying gene expression at single-molecule resolution.

3. The review discusses the challenges and prospects for single mRNA imaging applications using RNA aptamers: SFs systems, inspiring researchers to further develop these probes for cellular studies.

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