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1. Biosynthesis using CO2 as a carbon source is receiving attention for its potential to replace fossil fuels and carbohydrates in manufacturing, but it has limitations such as low driving force and limited CO2 fixation rate.

2. An approach to overcome these limitations is to combine biological and chemical syntheses, such as using energy-rich one-carbon compounds like methanol and formate in microbial metabolism or achieving CO2 fixation in cell-free synthesis.

3. The authors report on an engineered chemoenzymatic system that efficiently converts CO2 and methanol into the amino acid glycine, based on an engineered reversible glycine cleavage system with biocompatible electron-shuttle chemistry and protein engineering to enhance performance. This system could potentially be used for the biosynthesis of other amino acids and derived products from air.

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