1. This study examined the effects of increased Ni doses on Theobroma cacao plants, and found that Ni toxicity was induced at a dose of 300 mg/kg in soil.
2. Ni toxicity caused changes in photosynthesis, antioxidant activity, transcriptional levels, and translocation from roots to shoots.
3. Plant defense mechanisms such as metallothioneins and amino acids were activated to reduce the toxic effects of Ni.
The article is generally reliable and trustworthy, providing a comprehensive overview of the effects of increased Ni doses on Theobroma cacao plants. It provides detailed information on the physiological changes observed in response to different concentrations of Ni, as well as the plant defense mechanisms activated to reduce its toxic effects. The article also cites relevant literature to support its claims and conclusions.
However, there are some potential biases that should be noted. For example, the study only focuses on one species of plant (Theobroma cacao), so it is unclear whether similar results would be observed with other species or varieties of plants. Additionally, while the article does discuss potential risks associated with increased Ni concentrations in soils, it does not provide any recommendations for how these risks can be mitigated or avoided. Finally, while the article does cite relevant literature to support its claims and conclusions, it does not explore any counterarguments or alternative perspectives that may exist in this field of research.