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Article analysis:

The article is generally reliable and trustworthy, as it is based on existing knowledge and research from other studies, such as Khan et al., Oh et al., and Wu et al. The authors provide detailed information about their method, including the resources used, the background of the experiment, and the results obtained. Furthermore, they provide a specification table that outlines the subject area and more specific subject area of their method.

However, there are some potential biases in the article that should be noted. For example, while the authors mention that their method can be performed with minimal resources, they do not provide any evidence or data to support this claim. Additionally, while they discuss how their protocol provides quantitative analysis of 2D and 3D imaging process at cellular and sub-cellular level, they do not explore any counterarguments or alternative methods that could be used to achieve similar results. Furthermore, while they discuss how incubation of root segments with Na+ probe (CoroNa-Green AM) provides better resolution in imaging process, they do not mention any possible risks associated with this procedure or any potential side effects it may have on the plants being studied.

In conclusion, while this article is generally reliable and trustworthy due to its detailed description of the method used by the authors as well as its references to other studies in the field, there are some potential biases that should be noted when evaluating its trustworthiness and reliability.