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Article summary:

1. GC-MS is a widely used and efficient analytical platform for metabolomics research due to its robust and reproducible nature, as well as the availability of well-established metabolite databases.

2. This review focuses on recent developments in GC-MS based metabolomics applications, with an emphasis on sample preparation and preservation techniques. It also discusses various chemical derivatization techniques and highlights alternate mass analyzers and newly reported GC columns suited for metabolomics.

3. The review also covers multidimensional GC-MS and its application in environmental and biomedical research, as well as the importance of bioinformatics in metabolomics studies. The aim is to provide an update on GC-MS analytical techniques for those starting in this field, highlighting key steps and common pitfalls to be aware of.

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